Methylene Blue

Materials:

• Methylene Blue
• Absolute Ethanol
• Potassium Hydroxide
• Deionized Water

Supplies:

• 4 x 1 litre bottle with screw-top lid
• 1000 mL graduated cylinder
• 4000 mL Erlenmeyer flask
• Scale
• Stirbar
• Magnetic stirrer
• Filter paper
• Funnel
• Gloves
• Dust/Mist respirator
• Apron
• Safety goggles or eye wear

Method Stepwise:

1. Put on personal safety apparel.
2. Weigh out 9.0 g of Methylene Blue and put into the 4000 mL Erlenmeyer flask.
3. Add 900 ml of Absolute Alcohol.
4. Weigh out 0.3 g of Potassium Hydroxide and add to the Erlenmeyer flask.
5. Add 3000 mL of Deionized Water.
6. Add a stirbar to the flask and place onto a magnetic stirrer.
7. Allow the solution to dissolve for 2 to 3 days.
8. When dissolved filter into four one-litre bottles.
9. Label the bottles with the required information (i.e. name of solution, date made, expiry date, preparer's initials).
10. Store at room temperature for up to one year.